Antioxidant constituents from Smilax riparia / 中国中药杂志

By repeated column chromatography, including silica gel, toyopearl HW-40 and preparative HPLC, thirteen compounds (1-13) were isolated and purified from Smilax riparia. On the basis of spectral data analysis, the structures of isolated compounds were elucidated as 5-methoxy-[6]-gingerol (1), dehydroabietic acid (2), pteryxin (3), 2-methylphenyl-1-O-beta-D-glucopyranoside (4), 3,5-dimethoxy-4-hydroxybenzonic acid (5), isovanillin (6), vanillic acid (7), p-hydroxycinnamic acid (8), p-hydroxycinnamic methyl ester (9), p-hydroxybenzaldehyde (10), ferulic acid methyl ester (11), benzoic acid (12) and 5-hydroxy-methyl-2-furalclehyde (13). Compounds 1-4 and 8-12 were isolated from this genus for the first time. All compounds were isolated from this plant for the first time. Compounds 1 and 5-11 showed antioxidant activities on DPPH method.

Chemical constituents of Phymatopteris hastate and their antioxidant activity / 中国中药杂志

<p><b>OBJECTIVE</b>To study chemical constituents contained in Phymatopteris hastate and their antioxidant activity.</p><p><b>METHOD</b>Chemical constituents were separated and purified from P. hastate by using such methods as silica gel, Toyopearl HW-40C and HPLC preparative chromatography. Their structures were identified by spectroscopic methods such as NMR. Furthermore, 1, 1-diphenyl-2-picryl-hydrazyl(DPPH) method was used to assess the antioxidant activity of each compound.</p><p><b>RESULT</b>Fourteen compounds were separated and identified as 4-O-beta-D-glucopyranosyl-ethyl-trans-caffeicate (1), kaempferlo-7-O-alpha-L-rhamnopyranside (2), kaempferol-3, 7-di-O-alpha-L-rhamnopyranoside (3), kaempferol-3-O-alpha-L-arabinofuranosyl-7-O-alpha-L-rhamnopyranoside (4), juglanin (5), naringin (6), naringenin-7-O-beta-D-glucopyranoside (7), trans-caffeic acid (8), trans-caffeic acid-3-O-beta-D-glucopyranoside (9), trans-cinnamic acid-4-O-beta-D- glucopyranoside (10), trans-melilotoside (11), cis-melilotoside (12), ethyl chlorogenate (13), protocatechuic acid (14). The antioxidation experiment showed an obvious antioxidant activity in compounds 1-9, 13-14.</p><p><b>CONCLUSION</b>All of the compounds were separated from this genus for the first time. Among them, compound 1 was not seen in literature reports and assumed to be a new artifact derived from compound 9 and ethanol. Compounds 1-9, 13-14 showed a remarkable antioxidant activity.</p>

Chemical constituents contained in Tetraena mongolica / 中国中药杂志

To study chemical constituents contained in Tetraena mongolica. Chemical constituents were separated and purified by using such methods as silica gel, Toyopearl HW-40C and HPLC preparative chromatography. Their structures were identified by organic spectral method. One new compound was separated from T. mongolica and identified olean-11-oxo-12-en-28-ol-3beta-yl-caffeate.

Studies on anti-tumor metastatic constituents from Lindera glauca / 中国中药杂志

<p><b>OBJECTIVE</b>To study the anti-tumor metastatic constituents from Lindera glauca.</p><p><b>METHOD</b>Constituent isolation and purification was carried by repeated column chromatography (silica gel, Toyopearl HW-40 and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The anti-tumor metastasis assay was applied to evaluate the isolated compounds of their activities.</p><p><b>RESULT</b>Ten compounds (1 - 10) were isolated and their structures were identified by comparison of their spectral data with literature values as follows: Laurotetanine (1), N-methyllaurotetanine (2), reticuline (3), pallidine (4), N-trans-feruloyltyramine (5), N-cis-feruloyltyramine (6), atheroline (7), norisosocorydine (8), [9,9,9-(2) H3]-(1S*, 3S*, 4S*, 8S*)-p-menthane-3,8-diol (9), [9,9,9-(2) H3 ]-(1S*, 3R*, 4S*, 8S*)-p-Menthane-3,8-diol (10). Compounds 1, 2, 4, 5, 7 and 9 showed positive anti-tumor metastatic activities,and compounds 1, 4, and 5 showed significant anti-tumor metastatic activities.</p><p><b>CONCLUSION</b>Compound 3 was isolated from this plant for the first time. Compounds 9 and 10 were isolated from Lindera genus for the first time. Compounds 1, 4, and 5 showed significant anti-tumor metastatic activities.</p>

Studies on anti-tumor metastatic constituents from Ardisia Crenata / 中国中药杂志

<p><b>OBJECTIVE</b>To study the anti-tumor metastatic constituents from Ardisia Crenata.</p><p><b>METHOD</b>Chemical constituents were isolated and purified by repeated column chromatography( silica gel, Toyopearl HW40C and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The anti-tumor metastasis assay was applied to evaluate the isolated compounds of their activities.</p><p><b>RESULT</b>Nine compounds(1-9) were isolated and their structures were identified by comparison of their spectral data with literature values as follows: 5-hydroxymethyl-2-furalclehyde(1), ethyl-beta-D-fructopyranoside(2), syringic acid(3), n-butyl-beta-D-fructofuranoside(4), n-butyl-alpha-D-fructofuranoside(5), methyl-alpha-D-fructofuranoside(6), (+)-bergenin(7), ardisiacrispins B(8), asperuloside acid(9). The isolated compounds(1-9) showed positive anti-tumor metastatic activities, and compounds 1, 5, and 8 showed significant anti-tumor metastatic activities. At the concentration of 0.8 mg x L(-1), compound 5 revealed the value of metastatic inhibition ratio on MDA-MB-231 was 93.8%.</p><p><b>CONCLUSION</b>Compounds 2-6 and 9 were isolated from this plant for the first time. compounds 1, 5 and 8 showed significant anti-tumor metastatic activities.</p>

Phenol constituents of Pachysandra terminalis and their antioxidant activity / 中国中药杂志

<p><b>OBJECTIVE</b>To study the phenol constituents from Pachysandra terminalis and their antioxidant activities.</p><p><b>METHOD</b>Constituent isolation and purification was carried by repeated column chromatography (silica gel, Toyopearl HW-40 and preparative HPLC), and their structures were elucidated on the basis of spectral data analysis. DPPH method was used to evaluate the free radical scavenging activity of the isolated compounds.</p><p><b>RESULT</b>Nine phenol compounds (1-9) were isolated and their structures were identified as follow: p-hydroxybenzaldehyde (1), vanillin (2), 1-(4-hydroxy-3-methoxyphenyl) -ethanone (3), syringaldehyde (4), salicylic acid (5), p-hydroxybenzoic acid (6), ferulic acid (7), 2,3,4-trihydroxybenzoic acid (8), 3,4-dihydroxybenzoic acid (9). The isolated compounds showed obviously antioxidant activity. At the concentration of 50 micromol x L(-1), compounds 7-9 revealed DPPH free radical scavenging rates were 87.8%, 97.8% and 92%, respectively.</p><p><b>CONCLUSION</b>Compounds 1-9 were isolated from this genus for the first time. They showed the significant antioxidant activity.</p>

Immunosuppressive triterpenes from Tetraena mongolica / 中国中药杂志

<p><b>OBJECTIVE</b>To study the immunosuppressive constituents from Tetraena mongolica.</p><p><b>METHOD</b>Chemical constituents were isolated and purified by repeated column chromatography( silica gel, Toyopearl HW40C and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The MTT assay was applied to evaluate the isolated compounds on the inhibition effect of lymphocyte transformation.</p><p><b>RESULT</b>Six triterpenes were isolated and their structures were identified as follows: 3beta-hydroxy-11alpha, 12alpha:13beta,28-diepoxyoleanane(1), 3beta-(3, 4-dihydroxycinnamoyl)-erythrodi-ol(2), olean-28-al-3beta-yl-caffeate(3), erythrodiol (4), 12-oleanaen-3beta-caffeate(5), 3-O-(E) -coumaroylerythrodiol(6). Compound 24 exhibited the inhibition effects on lymphocyte transformation.</p><p><b>CONCLUSION</b>Compounds 1-6 were isolated from this plant for the first time, and compound 1 was a new nature product. Compound 2-4 showed significant immunosuppressive activity.</p>

Chemical constituents from roots of Phlomis umbrosa / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Phlomis umbrosa.</p><p><b>METHOD</b>Chemical constituents were isolated by repeated column chromatography (Toyopearl HW-40C and preparative HPLC). The structures were elucidated on the basis of spectral data analysis.</p><p><b>RESULT</b>Thirteen compounds were identified as oleanolic acid (1), corosolic acid (2), hederagenin (3), arjunolic acid (4), belleric acid (5), 3beta-hydroxy-29-al-12-en-28-oleanoic acid (6), butyrospermol (7), beta-sitosterol (8), daucosterol (9), caffeic acid (10), vanillic acid (11), p-hydroxy-benzoic acid (12), 3, 4-dihydroxy-benzoic acid (13).</p><p><b>CONCLUSION</b>Compounds 3 9, 11-13 were isolated from the plant for the first time.</p>

Constituents from Alternanthera philoxeroides and their antitumor activity / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate antitumor constituents from n-butyl alcohol extract of Alternanthera philoxeroides.</p><p><b>METHOD</b>The constituents were isolated with silica gel, gel permeation chromatography, and purified by HPLC. Their structures were elucidated by spectroscopy and acid hydrolysis. The antitumor effects of extracts and isolated compounds were tested by MTH method in vitro.</p><p><b>RESULT</b>Seven compounds were isolated and elucidated as followings: oleanolic acid 3-O-beta-D-glucuronopyranoside (1), oleanolic acid 28-O-beta-D-glucopyranoside (2), oleanolic acid 3-O-beta-D-glucuronopyranoside-6'-O-methyl ester (3), chikusetsusaponin IV a methyl ester (4), hederagenin 3-O-beta-D-glucuronopyranoside-6'-O-methyl ester (5), 4,5-dihydroblumenol (6), 6S,7E,9R-6,9-di-hydroxymegastigma-4,7-dien-3-one-9-O-beta-D-glucopyranoside (7). Compound 1 showed significant inhibitory effect against Hela and L929 with inhibitive ratios 91.3% and 92.9% at 30 mg x L(-1), respectively.</p><p><b>CONCLUSION</b>Compounds 4, 5 and 7 were isolated from this genus for the first time. Compound 1 showed significant inhibitory effect against Hela and L929 at 30 mg x L(-1).</p>

Chemical consitituents from Onychium japonicum / 中草药

Objective To study the chemical constituents from Onychium japonicum.Methods Che-mical constituents were isolated by repeated column chromatography and preparative HPLC,and their structures were elucidated on the basis of spectroscopic method.Results Ten compounds were identifed as:4,3',4'-trihydroxy-2,6-dimethoxychalcone(1),chrysoeriol(2),luteolin(3),butin(4),protocatechuic acid(5),3,4-dihydroxy-acetophenone(6),caffeic acid(7),vanillic acid(8),2,4-dihydroxybenzaldehyde(9),syringic acid(10),and ?-sitosterol(11).Conclusion Compound 1 is a new product named japonicone D,compounds 2-10 are isolated from the plants of Onychium Kaulf for the first time.

Diterpenes from Tripterygium wilfordii and their anti-cancer activities / 中草药

Objective To study the anti-cancer constituents from Tripterygium wilfordii. Methods Chemical constituents were isolated by repeated column chromatography (silica gel, Toyopearl HW-40 C and preparative HPLC), their structures were elucidated on the basis of spectroscopic methods, and the anti-cancer activity was screened by MTT method. Results Five diterpenes, 3-epi-triptobenzene B (Ⅰ), 3?, 14-dihydroxy-abieta-8, 11, 13-triene (triptobenzene B,Ⅱ), wilforol E (Ⅲ), triptohairic acid (Ⅳ), and 11-hydroxy-14-methoxy-18(4→3)-abeo-abietan-3, 8, 11, 13-tetraen-18-oic-acid (hypoglic acid, Ⅴ) were isolated from T. wilfordii. Conclusion Compound Ⅰ is a new compound named as triptobenzene L, compound Ⅳ is isolated for the first time. The compounds Ⅰ-Ⅴ show the positive anti-cancer effects on HeLa and L929 cell lines.

Antivirus constituents from Alternanthera philoxeroides / 中草药

Objective To investigate the anti-HBV constituents from Alternanthera philoxeroides.Methods The constituents were isolated with silica gel and gel permeation chromatography,and purified by HPLC.Their structures were elucidated by spectroscopy.The antivirus effects of the isolated compounds were tested by ELISA method in vitro.Results Ten compounds were isolated and elucidated as followings:oleanolic acid(Ⅰ),oleanolic acid 3-O-?-D-glucuronopyranoside(Ⅱ),oleanolic acid 28-O-?-D-glucopyranoside(Ⅲ),chikusetsusaponin Ⅳ a methyl ester(Ⅳ),4,5-dihydroblumenol(Ⅴ),N-trans-feruloyl 3-methyldopamine(Ⅵ),N-trans-feruloyl tyramine(Ⅶ),3?-hydroxystigmast-5-en-7-one(Ⅷ),24-methylenecycloartanol(Ⅸ),and cycloeucalenol(Ⅹ).The values of inhibition percent of compounds Ⅰ-Ⅲ,Ⅴ-Ⅶ revealed a significant distinction compared to the control group.Compounds Ⅱ and Ⅴ showed significant inhibition against HepG2 cells transected with cloned hepatitis B virus DNA,their inhibitive ratios were 85.38% and 87.37% at 50 ?g/mL,respectively.Conclusion Compounds Ⅳ-Ⅶ are isolated from this plant for the first time and phenolic amides have been determined as the new structure type from the plants of Alternanthera Forsk.Compounds Ⅱ and Ⅴ from A.philoxeroides show the more significant anti-HBV activities.

Diterpenoids possessed immunosuppressive activity from Tripterygium hypoglaucum / 中草药

Objective To study the diterpenoids possessed immunosuppressive activity from Tripterygium hypoglaucum. Methods The chemical constituents were isolated from the CHCl3 extracts of T. hypoglaucum by repeated column chromatography, including silica gel, Sephadex LH-20, and preparative HPLC. Their structures were elucidated on the basis of spectroscopic studies. The immunosuppressive activity of these compounds was tested using the lymphocyte transformation test. Results Compounds Ⅰ-Ⅵ were identified as triptobenzene H (Ⅰ), triptoquinone A (Ⅱ), triptoquinone B (Ⅲ), triptoquinone H (Ⅳ), triptonediol (Ⅴ), triptonoterpene (Ⅵ). Conclusion Compound Ⅰ-Ⅲ are isolated from this medicinal plant for the first time and all the compounds show the significant immunosuppressive activity.

Quantitative determination of triptoquinone B in Radix Folium Seu Flos Tripterygii Wilfordii and its tablets by HPLC / 中草药

Objective To establish an HPLC method for determination of triptoquinone B in Radix Folium Seu Flos Tripterygii Wilfordii(RFFTW) and its tablets.Methods An external method with HiQ siL KYA-C_(18)(250 mm?4.6 mm,5 ?m) column as fixed phase and methanol-1% acetic acid solution((66∶)34) as mobile phase was adopted.The detective wave length was 254 nm and the flow rate was 1.0 mL/min.Results The linearity range was 6.62—105.9 ?g/mL(r=0.999 9),the average recovery of T.wilfordii from Hunan Province was 97.78%,RSD was 1.09%(n=9),and the average recovery of tablet of T.hypoglaucum was 99.83%,RSD was 1.76%(n=9).Conclusion The method is accurate and sensitive.It is adoptable for quantitative analysis of triptoquinone B in RFFTW and its tablets.

Determination of tripterine in Tripterygium wilfordii and its tablets by HPLC / 中草药

Objective To determine the content of tripterine in Tripterygium wilfordii and its tablets by HPLC.Methods An external standard method by HPLC with Zorbax C_(18)column as fixed phase and methanol-1% HAc(87∶13) as mobile phase was adopted.The detection wavelength was 425 nm and the flow rate was 1.0 mL/min.Results The linear range for tripterine was 40.96～204.8 ?g/mL(r=(0.999 6).) The average recovery of Chinese medicinal materials was 98.37% and RSD was 1.01%(n=9);the average recovery of preparation sample was 98.59% and RSD was 1.18%(n=9).Conclusion The method is simple and accurate,which can be adoptable for quantitative analysis of tripterine in the plants of Tripterygium L.

Determination of triptoquinone H in Tripterygium wilfordii and its Tablet by HPLC / 中草药

Objective To develop an HPLC method for determination of triptoquinone H in Tripterygium wilfordii and its Tablet preparations. Methods An external method with Agilent Zorbax SC-C_(8)(250 mm?4.6 mm, 5 ?m) column as fixed phase and methanol-water (75∶25) as mobile phase was adopted. The detective wavelength was 258 nm and the flow rate was 1.0 mL/min. Results The linearity range of triptoquinone was 6.28 — 100.5 ?g/mL (r=0.999 7). The average recovery of T. wilfordii was 96.94%, RSD was 1.57% (n=9) and the average recovery of T. hypoglaucum Tablet was 100.02%, RSD was 1.74% (n=9). Conclusion The method is accurate and sensitive. It is adoptable for quantity analysis of triptoquinone H in T. wilfordii and its Tablet.

A new quinolone alkaloid with antibacterial activity from Lappula echinata / 中草药

Objective To investigate the chemical constituents of Lappula echinata and determine the antibacterial activity.Methods A new quinolone alkaloid was isolated from the BuOH extract of L.echinata by silica gel column chromatography and gel column chromatography.Its structure was identified by 1H-NMR, 13C-NMR, 2D-NMR, HR-MS, UV, and IR spectral data analysis.Its antibacterial activity was determined by KB method.Results A new quinoloe alkaloid named 8-methoxy-4-quinolone-2-caboxylic acid was isolated from L.echinata and was found to have antibacterial activity on Pseudomonas pyocyanea ATCC 27853, EPEC O111, pneumobacillus and Staphylococcus epidermidis.Conclusion This is a new compound with antibacterial activity.